The study of the polysaccharide composition of the herb of Alchemilla L. genus species growing in the territory of the Precarpathian region

Medicines of plant origin containing polysaccharides are used in pharmaceutical practice since they exhibit a wide spectrum thepharmacological activity. Species the Alchemilla L. genus Rosaceae family important scientific and practical importance; contain different groups biologically active substances (BAS), including phenolic compounds polysaccharides. The lack information literature on quantitative content this raw material indicates topicality research direction. Aim. To isolate study polysaccharide composition herb species growing territory Precarpathian region. Materials methods. fractions their monomer composition, we 6 (Alchemilla (A.) flabellata Buser., A. subcrenata phegophila Juz., microdonta hebescens turkulensis Pawі.) harvested during mass flowering phase various areas Ivano-Frankivsk region within 2020-2021. studied was determined by gravimetric method after successive extraction with purified water R, hydrochloric acid solution sodium hydroxide solution, followed precipitation 96 % ethanol R. qualitative ascending paper chromatography(PC) thin-layer chromatography (TLC) solvent systems compared to authentic samples neutral acidic monosaccharides. Results discussion. It found that total ranged from 7.73 15.35 %, depending type species. yield water-soluble (WSP) 2.62 5.49 pectin (PS) – 1.41 2.13 hemicellulose (HC) A 0.45 2.96 HC B 2.51 6.44 %. maximum amount WSP observed Pawł. (5.49 respectively), highest PS detected Juz. (2.13 respectively). monosaccharides methods PC TLC samples. Glucose arabinose were identified hydrolysates represented glucose, galactose. Glucose, galactose xylose hydrolyzates fraction; galactose, arabinose, xylene, rhamnose, glucuronic galacturonic acids fraction. Conclusions. For first time, complexes have been isolated sugars has determinedby WSP, PS, results obtained importance for further pharmacological activity can be development quality control medicinal it.